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Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid? International Journal of Morphology
Hipkaeo,Wiphawi; Suzuki,Ryoji; Abe,Hiroshi; Chaisiwamongkol,Kowit; Kondo,Hisatake.
After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron...
Tipo: Journal article Palavras-chave: Glomerulus; Slit; Nephrin; Localization; Immunohistochemistry.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016
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Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ International Journal of Morphology
Hipkaeo,Wiphawi; Techataweewan,Nawaporn; Sakaew,Waraporn; Maleewong,Wanchai; Sripa,Banchob; Chomphoo,Surang; Hozumi,Yasukazu; Goto,Kaoru; Kondo,Hisatake.
By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the...
Tipo: Journal article Palavras-chave: DGK<; Img border=0 width=17 height=33 src="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg" alt="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg">; Immunoreactivity Peripheral neurons Glia Opisthorchis viverrini Ultrastructure.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200010
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Localization of Cannabinoid Receptor 1 (CB1) in Submandibular and Sublingual Salivary Glands of Mice throughout Postnatal Development International Journal of Morphology
Hipkaeo,Wiphawi; Watanabe,Masahiko; Kondo,Hisatake.
In view of the fact that human marijuana users often show dry mouth symptom, the present study was attempted to examine the localization of CB1, which was originally identified in brain, in the submandibular and sublingual salivary glands of postnatal developing male mice by immunohistochemistry. In submandibular gland, CB1-immunoreactivity was positive in a majority of acinar cells in forms of granular appearance in their apical cytoplasm, while it was negative in the ducts at newborn stage. The immunoreactivity decreased in the acinar cells at P1W and no immunoreactivity was detected in the acinar cells at P3W and thereafter. The immunoreactivity was positive in ductal cells at P3W and it remained positive thereafter until P8W stage. The immunoreaction...
Tipo: Journal article Palavras-chave: Cannabinoid receptor 1; Immunohistochemistry; Submandibular gland; Sublingual gland; Postnatal mice.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200044
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Localization of Vesicular Inhibitory Amino Acid Transporter (VIAAT) in the Submandibular Salivary Gland Throughout the Postnatal Development of Mice International Journal of Morphology
Toomsan,Yanyong; Chomphoo,Surang; Pakkarato,Sawetree; Watanabe,Masahiko; Kondo,Hisatake; Hipkaeo,Wiphawi.
According to recent studies, it is highly possible that the occurrence of vesicular inhibitory amino acid transporter (VIAAT) is a good marker of GABA-signaling not only in the brain, but also in extra-brain tissue cells containing GABA and GAD. In view of this, the present study was attempted to localize VIAAT-immunoreactivity in the submandibular gland of mice. In the present study, the submandibular glands of male mice at various postnatal developmental stages were examined for detailed localization of VIAAT-immunoreactivity in immunohistochemistry at light microscopic level. The immunoreactivity for VIAAT was localized in epithelial cells of proximal and distal excretory ducts with the striated portion more intensely immunopositive at young postnatal...
Tipo: Journal article Palavras-chave: VIATT-immunoreactivity; GABA; Salivary glands; Arteriolar smooth muscle.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100019
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New and Probable Ultrastructural Criteria for Identification of Cytoplasmic Sol ­ Gel States International Journal of Morphology
Hipkaeo,Wiphawi; Kondo,Hisatake.
In embedment-free transmission electron microscopy without employing epoxy embedding media, the cytoplasmic matrix, in which cell organelles and elements including the cytoskeletons are held in place, lattices of strands are clearly and constantly disclosed in every cell. Their compactness is variable in different kinds of cells and in different domains of one and the same cell, and it is changeable under hypo- or hyper-osmolarity. In addition, the appearance of strand-lattices is duplicable in artificial proteins at different sol/gel states and concentrations. All taken together, a new and probable ultrastructural criteria has been proposed for identification of cytoplasmic sol/gel states with a hope that the dynamic properties of the cell is understood...
Tipo: Journal article Palavras-chave: Sol and gel; Cytoplasm; Embedment-free; Ultrastructure.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100030
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Sexual Dimorphism Using the Interstyloid Distances and Clinical Implication for Elongated Styloid Process in Northeastern Thailand International Journal of Morphology
Sakaew,Waraporn; Arnanteerakul,Tansita; Somintara,Somsuda; Ratanasuwon,Somsiri; Uabundit,Nongnut; Iamsaard,Sittichai; Chaisiwamongkol,Kowit; Chaichun,Amnart; Hipkaeo,Wiphawi.
Skeletal remains are crucial in forensic identification of the sex, especially human skulls including the styloid process, a bony projection from the skull. Hence, the objectives of the present study were undertaken to assess the value of the styloid process for the sex identification of unknown skulls and also to investigate the prevalence of elongated styloid process in 102 human dry skulls from the northeast Thai population. As a result, the interstyloid distances at both base and tip of the styloid processes were found to be significantly different between male and female specimens, although no significant difference was found in the length of the styloid process between males and females. In addition, the occurrence of the elongated styloid process...
Tipo: Journal article Palavras-chave: Styloid process; Elongated styloid process; Interstyloid distance; Sex identification.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400008
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Types of Facets on the Superior Articular Surface of Isan-Thai Dried Calcanei International Journal of Morphology
Iamsaard,Sitthichai; Uabundit,Nongnut; Boonruangsri,Porntip; Sawatpanich,Tarinee; Hipkaeo,Wiphawi.
The patterns of talar articulating facets must be concerned in surgical procedure or the internal and external fixation in various diseases of the foot. The variant types of calcaneal facets on the superior articular surface have been reported in many races except in Thais. This study therefore was aimed to investigate the patterns of superior articulating facet of dried calcanei in Isan-Thais. The identified 396 Isan- Thai dried calcanei (202 males and 194 females) were carried out for variant superior facet observations. The results showed that types of facets observed could be classified into three major types (Type 1 [60.86%], Type 2 [38.64%], and Type 4 [0.51%], respectively). In sub-type classifications, there were Type 1A (24.75%), Type 1B (36.11%),...
Tipo: Journal article Palavras-chave: Types; Talar articulating facets; Isan-Thai calcanei.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400058
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Version of Femoral Neck: A Cadaveric Dried Bone Study International Journal of Morphology
Khamanarong,Kimaporn; Hipkaeo,Wiphawi; Chatuparisute,Piyawan; Sae-Jung,Surachai; Tepsutamarat,Kaewjai.
The aim of this study was to measure the version of femoral neck on dried Thai human femora. The version of femoral neck varies widely. It is important to know the version of femoral neck in a particular population to undertake successful femoral neck reconstructive surgery. Paired 216 dried femora of adult Thais from the bone collection maintained in the Department of Anatomy at the Faculty of Medicine, Khon Kaen University, Thailand, were used to measure the version of femoral neck. The mean+SD femoral anteversion (FNA) was 16.21+5.24 degrees. The 95% confidence interval of FNA was from 15.48 to 16.94 degrees. The 95% confidence intervals of male and female average FNA were 14.75 to 16.90 and 15.59 to 17.59 degrees respectively. There was no significance...
Tipo: Journal article Palavras-chave: Version femoral neck; Dried femora; Reconstructive surgery.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200015
Registros recuperados: 8
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